This book provides a comprehensive volume that integrates a wide spectrum of methods required to implement current and future progress in tissue engineering. Leading experts in various disciplines provide examples of recent advances in tissue engineering-related methodologies. Authors present authoritative discussions on topics. The book supplies an understanding of diverse technologies and methods used to drive tissue engineering into a clinical reality. The knowledge contained in this volume represents the impressive progress made in the decade when tissue engineering rapidly evolved.

Contents Contributors In Vitro Expansion of Tissue Cells by Conditional Proliferation Tobias May, Hansjörg Hauser, and Dagmar Wirth Stem Cell Engineering using Transducible Cre Recombinase Lars Nolden, Frank Edenhofer, Michael Peitz, and Oliver Brüstle Human Embryonic Stem Cells for Tissue Engineering Daniel Kitsberg Culture and Characterisation of Human Bone Marrow Mesenchymal Stem Cells Bruno Delorme and Pierre Charbord Skeletal ("Mesenchymal") Stem Cells for Tissue Engineering Pamela Gehron Robey, Sergei A. Kuznetsov, Mara Riminucci, and Paolo Bianco Biomaterials/Scaffolds Design of Bioactive, Multiphasic PCL/collagen Type I and Type II - PCL-TCP/collagen Composite Scaffolds for Functional Tissue Engineering of Osteochondral Repair Tissue by Using Electrospinning and FDM Techniques Detlef Schumann, Andrew K. Ekaputra, Christopher X.F. Lam, and Dietmar W. Hutmacher Synthetic Hydrogel Matrices for Guided Bladder Tissue Regeneration Catharina A.M. Adelöw, and Peter Frey Generation of Multicellular Tumour Spheroids by the Hanging Drop Method Nicholas E. Timmins and Lars K. Nielsen In-vitro Vascularization of Human Connective Microtissues Jens M. Kelm, Wolfgang Moritz, Doerthe Schmidt, Simon P. Hoerstrup, and Martin Fussenegger Artificial Skin Matthias Föhn and Holger Bannasch Small Blood Vessel Engineering Patrick Au, Josh Tam, Dai Fukumura, and Rakesh K Jain Artificial Pancreas to Treat Type 1 Diabetes Mellitus Riccardo Calafiore and Giuseppe Basta Human Articular Chondrocytes Culture Andrea Barbero and Ivan Martin Cardiomyocytes from Human and Mouse Embryonic Stem Cells Christine Mummery, Marcel A.G. van der Heyden, Teun P. de Boer, Robert Passier, Dorien Ward, Stieneke van den Brink, Marga van Rooijen, Anja van de Stolpe Myocardial Restoration and Tissue Engineering of Heart Structures Theo Kofidis, Knut Müller-Stahl, and Axel Haverich Practical Aspects of Cardiac Tissue Engineering with Electrical Stimulation Christopher Cannizzaro, Nina Tandon, Elisa Figallo, Hyoungshin Park, Sharon Gerecht, Milica Radisic, Nicola Elvassore, and Gordana Vunjak-Novakovic Biological Scaffolds for Heart Valve Tissue Engineering Artur Lichtenberg, Serghei Cebotari, Igor Tudorache, Andres Hilfiker, and Axel Haverich In Vitro Heart Valve Tissue Engineering Dörthe Schmidt, Anita Mol, Jens M. Kelm, and Simon P. Hoerstrup