Continuing the research of the best-selling first edition, this second edition collects three more years of research in the ever-expanding study of the cell membrane. It covers the latest developments in the "traditional" patch techniques. This authoritative second edition updates the standard techniques while introducing three brand new, cutting-edge technical advances in the field. Thorough and timely, this edition is an invaluable resource.

Leseprobe

TABLE OF CONTENTS 1. Technology of Patch Clamp Electrodes James L. Rae and Richard A. Levis 2. Whole Cell Patch-Clamp Recordings Harald Sontheimer and Michelle L. Olsen 3. Single-Channel Recording David J.A. Wyllie 4. Combined Fluorometric and Electrophysiological Recordings Hartmut Schmidt and Jens Eilers 5. Combining Uncaging Techniques with Patch Clamp Recording and Optical Physiology Dmitry V. Sarkisov and Samuel S.-H. Wang 6. Visually-Guided Patch-Clamp Recordings in Brain Slices James R. Moyer, Jr. and Thomas H. Brown 7. In Vivo Patch-Clamp Technique Hidemasa Furue, Toshihiko Katafuchi and Megumu Yoshimura 8. Techniques for Perforated Patch Recordings Constantine Sarantopoulos 9. Fast-Drug Application Manfred Heckmann and Stefan Hallermann 10. Pipette Internal Perfusion: Methods and Applications Srinivas M. Tipparaju and Aruni Bhatnagar 11. Loose-Patch Clamp Method Hector G. Marrero and Jose R. Lemos 12. Recording Currents from Channels and Transporters in Macropatches Guiying Cui, Matthew D. Fuller, Christopher H. Thompson, Zhi-Ren Zhang and Nael McCarty 13. Structure-Function Analyses of Single Cells by Combining Patch-Clamp Techniques with RT-PCR Gerald Seifert and Christian Steinhauser 14. Planar Patch Clamping Jan C. Behrends and Niels Fertig 15. Automated Glass Pipette-based Patch Clamp Techniques Michael Fejtl, Uwe Czubayko, Alexander Hummer, Tobias Krauter and Albrecht Lepple-Wienhues