Major improvements in instrumentation and specimen preparation have brought SEM to the fore as a biological imaging technique. Although this imaging technique has undergone tremendous developments, it is still poorly represented in the literature, limited to journal articles and chapters in books. This comprehensive volume is dedicated to the theory and practical applications of FESEM in biological samples. It provides a comprehensive explanation of instrumentation, applications, and protocols, and is intended to teach the reader how to operate such microscopes to obtain the best quality images.

Table of Contents 1. Low Voltage Scanning Electron Microscopy In Biology--Historical Preface : The Early Development of the Scanning Electron Microscope to 1965...1 Dennis McMullan 2. LVSEM For Biology...27 James B. Pawley, PhD 3. The Aberration-Corrected SEM...101 David Joy Science and Engineering Research Facility, University of Tennessee 4. Noise and its effects on the Low Voltage SEM...121 David C Joy 5. High-Resolution Low Voltage Field-Emission Scanning Electron Microscopy (HRLVFESEM) Applications for Cell Biology and Specimen Preparation Protocols...146 Heide Schatten, PhD 6. Molecular labeling for Correlative Microscopy: LM, LVSEM, TEM, EF-TEM and HVEM...173 Ralph Albrecht and Daryl Meyer 7. Low kV and video-rate, beam-tilt stereo for viewing live-time experiments in the SEM...209 Alan Boyde 8. CryoSEM of Chemically Fixed Animal Cells...228 Stanley L. Erlandsen 9. High resolution and low voltage SEM of plant cells...249 Guy Cox, Peter Vesk, Teresa Dibbayawan, Tobias I. Baskin and Maret Vesk 10. High Resolution Cryo-Scanning Electron Microscopy of Biological Samples...268 Paul Walther 11. Developments in Instrumentation for Microanalysis in Low Voltage Scanning Electron Microscopy...305 Dale E. Newbury